Question: If You Look At The Structure Of Lipase, Usually Works Well At PH Of 7.7 And 37°C, You Will Sort Out That Changing The Reaction Medium PH Would Affect The Activity Of The Enzyme. This Is Not Surprising As The Substrate Attach Itself To The Enzyme Through Some Ionic Bonds, Shown In P1- A. Typically, Transfer Of A Hydrogen Ion From A -COOH Group In The …

If you look at the structure of lipase, usually works well at pH of 7.7 and 37°C, you will sort out that changing the reaction medium pH would affect the activity of the enzyme. This is not surprising as the substrate attach itself to the enzyme through some ionic bonds, shown in P1- a. Typically, transfer of a hydrogen ion from a -COOH group in the side chain of one amino acid residue to an -NH3 group in the side chain of another. The reaction scheme is shown in P1-b and you have been asked to: a) Prove the form of the modified Michales-Menten equation (Eql-a) by making the necessary assumptions, b) Propose 1 method at least to determine kinetic parameters and what are the required information you need to determine them. c) Clarify how would kinetic parameters would be affected if the pH got increased to 9. E+H Vmax[S] V= 1 K2 K2 KM (1 + [H*]K NH3 Ку cob KM EHS- EH + S + EH + P СОО NH3 н K EH (P1-a) (P1-b) (Eql-a)