Question: In This Experiment, DNA Fragments Produced From The Restriction Digest Were Circularized During Ligation And Then Transformed Into E. Coli DH5alpha/pir. The Transformed Cells Were Plated Onto LB And LB + Kan Plates And Incubated Overnight At 37 C And The Results Are Below. LB Plates Are On The Left And LB + Kan Plates Are On The Right. R+ Samples Are …

In this experiment, DNA fragments produced from the restriction digest were circularized during ligation and then transformed into E. coli DH5alpha/pir. The transformed cells were plated onto LB and LB + kan plates and incubated overnight at 37 C and the results are below. LB plates are on the left and LB + kan plates are on the right. R+ samples are the top row, R samples are plated on the middle row, and the bottom row is the R- samples: 1. Regarding the color of the colonies, are these results that were expected? Why or why not? 2. If both the LB and LB + kan plates were plated from the same samples of mutated S. marcescens, why is there a substantial difference in the amount of growth between the two types of plates? 3. Briefly describe the results on each of the plates and any conclusions you can draw from these results.