Question: Media is to be batch sterilized in a 100 liter fermenter before it is used to grow microorganisms…

Media is to be batch sterilized in a 100 liter fermenter before
it is used to grow microorganisms. Using 0.001 as the probability
of an unsuccessful sterilization, compute the time required for
sterilizationat 121⁰, 125⁰, 130⁰C, and 135⁰C.   Ignore
heat up and cool down. Data are below are for bacillus
stearothermophilus spores which is the standard data used in the
absence other microbe specific data. (put boxes around your
answers)

For Spores present in the media:

Spore concentration: 1 X 10⁶ spores/liter

E =    67.7 kcal/mol

A = 1 x 10^36.2 min-1