Question: MiRNA and siRNA control multiple levels of gene expression. The
first level to be understood was …
miRNA and siRNA control multiple levels of gene expression. The
first level to be understood was the
translational level where RISC can interfere with the RNA
translation at many different
checkpoints and steps.
In the nucleus an Argonauste
(Ago)-mi/siRNA complex can interfere with mRNA production at several points from chromati
modification to alternative splicing.The figure also shows another important process
regulated by mi/siRNA, which is DNA repair, but ignore that part for this
question.
A) Explain succintly how the Ago-mi/siRNA complex is targeted to
the gene/DNA region under its
regulation.
B) Explain how the Ago-mi/siRNA
complex can control chromatin modification and transition from eu to heterochromatin. Be
specific of the different classes of proteins that can get involved in this
process.
C) Propose a scenario for the regulation of
alternative splicing.
Show transcribed image text Cytoplasm Exon Exon Exon RNAi Alternative splicing Transcription elongation Argonaute Nucleus mi/siRNA DNA Chromatin modification DNA repair of double strand break Nature Reviews Genetics
Cytoplasm Exon Exon Exon RNAi Alternative splicing Transcription elongation Argonaute Nucleus mi/siRNA DNA Chromatin modification DNA repair of double strand break Nature Reviews Genetics
