Question: Please help me to better understand this
figure thoroughly and in very good detail. This figure i…
Please help me to better understand this
figure thoroughly and in very good detail. This figure is from
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC151565/
Show transcribed image text PubMed Central, Figure 6: Mol Biol Cell. 2003 Mar, 14(3): 858-870. doi: 10.1091/mbc.E02-08-0532 Google Chrome www.ncbi.nlm.nih.gov/pmc/articles/PMC151565/figure/F6/ 2.0 Blot: anti-Grb2 0.8 SERNA3 0.4 Blot: an-c-Cbl Time (min) C HeLa MIKK 1.2 10 0.8 0.4 으20 Time (min) Depletion of Grb2 inhibits EGFR internalization. (A) HeLa and PAE cells were transfected with three different siRNA targeted to human Grb2 or mock-transfected as described in MATERIALS AND METHODS. After 2 d, equal amounts of cells were lysed, lysates were electrophoresed, and the amount of Grb2 and c-Cbl (control) in lysates was determined by Western blotting. (B) HeLa cells were depleted of Grb2 using sRNA3 as in A, and the rate of125-EGF (1 ng/ml) intemalization was measured as in Figure 2. The data are averaged from four independent experiments and the enror bars represent SEs. (C) Mean intermalization 125I-EGF rates were measured in cells that were transfected with sRNA3 as in B or siRNAs targeted to MEKK3 or Eps15. The rates are expressed as percent to the internalization rate in mock-transfected cells. The data are averaged from three to four independent experiments and the error bars represent SEs. D) PAE EGFR cells were depleted of Grb2 using siRNA3 or transfected with MEKK3-targeted siRNA, and the rate of125-EGF (1 ng/ml) intermalization was measured as in B. The data represent the averaged values from three independent exper nents and the error bars represent SEs. The average reduction of endocvtoss rate constant ke by siRNA 3 n tese experiments was 75% 590 (E β2-YFP was transiently expressed in PAE/EGFR cells depleted or not depleted of Grb2. The cells were incubated with 2 ng/ml EGF-Rh at 4°C for 1 h. The percent of cellular EGF-Rh colocalized with coated pits was calculated as in Figure 4. The bars represent the average values from seven cells and the error bars represent SDs of the mean p0.01) Ask me anything 11:54 AM 4/17/2016
PubMed Central, Figure 6: Mol Biol Cell. 2003 Mar, 14(3): 858-870. doi: 10.1091/mbc.E02-08-0532 Google Chrome www.ncbi.nlm.nih.gov/pmc/articles/PMC151565/figure/F6/ 2.0 Blot: anti-Grb2 0.8 SERNA3 0.4 Blot: an-c-Cbl Time (min) C HeLa MIKK 1.2 10 0.8 0.4 으20 Time (min) Depletion of Grb2 inhibits EGFR internalization. (A) HeLa and PAE cells were transfected with three different siRNA targeted to human Grb2 or mock-transfected as described in MATERIALS AND METHODS. After 2 d, equal amounts of cells were lysed, lysates were electrophoresed, and the amount of Grb2 and c-Cbl (control) in lysates was determined by Western blotting. (B) HeLa cells were depleted of Grb2 using sRNA3 as in A, and the rate of125-EGF (1 ng/ml) intemalization was measured as in Figure 2. The data are averaged from four independent experiments and the enror bars represent SEs. (C) Mean intermalization 125I-EGF rates were measured in cells that were transfected with sRNA3 as in B or siRNAs targeted to MEKK3 or Eps15. The rates are expressed as percent to the internalization rate in mock-transfected cells. The data are averaged from three to four independent experiments and the error bars represent SEs. D) PAE EGFR cells were depleted of Grb2 using siRNA3 or transfected with MEKK3-targeted siRNA, and the rate of125-EGF (1 ng/ml) intermalization was measured as in B. The data represent the averaged values from three independent exper nents and the error bars represent SEs. The average reduction of endocvtoss rate constant ke by siRNA 3 n tese experiments was 75% 590 (E β2-YFP was transiently expressed in PAE/EGFR cells depleted or not depleted of Grb2. The cells were incubated with 2 ng/ml EGF-Rh at 4°C for 1 h. The percent of cellular EGF-Rh colocalized with coated pits was calculated as in Figure 4. The bars represent the average values from seven cells and the error bars represent SDs of the mean p0.01) Ask me anything 11:54 AM 4/17/2016
