Question: PLEASE HELP
MOLECULAR TOOLBOX
Using the molecular tools provided in the table, your job is to
cre…
PLEASE HELP
MOLECULAR TOOLBOX
Using the molecular tools provided in the table, your job is to
create a recombinant plasmid that contains the human insulin gene.
The recombinant plasmid will be verified via bacteria
transformation and ultimately tested in diabetic animals.
|
MOLECULAR TOOLS |
|
|
ampicillin resistance gene |
petri dishes with agar |
|
bacterial transformation |
petri dishes with agar & ampicillin |
|
DNA ligase |
plasmid |
|
DNA containing human insulin gene |
restriction enzymes |
|
E. coli |
|
I. Place
the molecular tools in the order they will be used to
successfully clone the human insulin
gene.
|
MOLECULAR TOOLS |
|||
|
1. |
6. |
||
|
2. |
7. |
||
|
3. |
8. |
||
|
4. |
9. |
||
|
5. |
|||
(Visited 4 times, 1 visits today)
