Question: Problem 1. Media is to be batch sterilized in a 100 liter fermenter before it is used to grow mic…

Problem 1. Media is to be batch sterilized in a 100 liter
fermenter before it is used to grow microorganisms. Using 0.001 as
the probability of an unsuccessful sterilization, compute the time
required for sterilization at 121?, 125?, 130?C, and 135?C. Ignore
heat up and cool down. Data are below are for bacillus
stearothermophilus spores which is the standard data used in the
absence other microbe specific data. (put boxes around your
answers)

For Spores present in the media:

Spore concentration: 1 X 10⁶ spores/liter

E = 67.7 kcal/mol

A = 1 x 10^36.2 min-1

Problem 2. You are trying to make an intelligent choice of which
of the 4 pairs of temperatures and sterilization times (from
problem 1) to use, one consideration is the effect of the
sterilization process on heat labile components in the growth
medium such as L-glutamine which can degrade as a function of time
and temperature. The equation for k_d, _LG for
L-Glutamine is below. Where E_LG is 10 kcal/mol and A is
1×10⁴ min-1. The equation for the degradation of
L-Glutamine is also below. If your media has a 0.01M concentration
of L-Glutamine before sterilization. Please compute the
concentration of L-Glutamine that remains after sterilization for
each of the 4 pairs of time and temperature. Please, then comment
on which pair may be the best for this particular sterilization
process.

THIS IS NOT AN EXPERIMENTAL QUESTION. CALCULATIONS REQUIRED FROM
THE GIVEN INFORMATION. NO ADDITIONAL INFORMATION IS AVAILABLE
THEREFORE IT DOESNT GET ANY CLEARER THAN THIS. (This is the 4th
time posting the same question, all I need is some help)

WHAT YOU SEE HERE IS ALL THE INFORMATION THE TEACHER GAVE AS
HOMEWORK. SOME HELP WOULD BE GREATLY APPRECIATED