Question: Questions 1 and 2 are based on
the following information:
Lactose permease of E coli
is a protei…
Questions 1 and 2 are based on
the following information:
Lactose permease of E coli
is a protein composed of a single polypeptide that is 417 amino
acids in length. Amino acid residue 64 is the amino acid glycine
and aspartic acid is amino acid residue number 68.
1. Which of these amino acids is closer to the carboxyl terminus
of the polypeptide?
a. glycine
b. aspartic acid
2. Which of these amino acid’s codon is closer to the 3’ end of
the mRNA?
a. glycine
b. acid
3. Consider the amino acid tryptophan (trp). What is the
sequence of the anticodon on the charged tRNA?
a. 5’-UGG-3’
b. 3’-UGG-5’
c. 5’-ACC-3’
d. 3’-ACC-5’
e. 5’-TGG-3’
f. 3’-TGG-5’
4. Which of the following statements best describes the function
of aminoacyl tRNA synthetase?
a. It attaches a specific amino acid to a tRNA molecule.
b. It helps tRNA synthesize proteins.
c. It provides the energy required to attach a specific amino
acid to a tRNA molecule.
d. It synthesizes tRNA molecules.
e. It catalyzes the formation of the peptide bond between amino
acids during translation.
5. Place the following events of translation in the order in
which they occur:
i. tRNAfMet binds in the P site
ii. RF2 binds in the A site
iii. EF-Tu helps tRNAPhe bind in the A site
iv. IF3 binds to the 30S ribosomal subunit
v. Peptide bond formation
vi. EF-G uses GTP to move the ribosome
vii. The 16S rRNA in the 30 S ribosomal subunit recognizes and
interacts with the Sine-Delgarno sequence in mRNA
viii. 50S subunit binds to 30S subunit
a. i, ii, iii, iv, v, vi, vii, viii
b. i, vii, viii, iv, ii, vi, iii, v
c. i, ii, iv, vii, viii, iii, v, vi
d. iv, vii, i, viii, iii, v, vi, ii
e. viii, vii, iv, iii, v, i, vi, ii
