Question: Suppose you are studying gene X in yeast using four different strains. Strain 1 is haploid and ha…
Show transcribed image text Suppose you are studying gene X in yeast using four different strains. Strain 1 is haploid and has the wild type allele of gene X. Strain 2 is haploid and has a missense mutation halfway through the coding sequence of gene X. Strain 3 is haploid and has a nonsense mutation halfway through the coding sequence of gene X. Strain 4 is diploid and is heterozygous for the nonsense mutation in gene X (i.e.. it carries the wild type allele of gene X on one chromosome and the nonsense allele of gene X on the other homologue). You have a DNA probe that will hybridize with gene X and its mRNA transcript on southern and northern blots, respectively. You also have an antibody that will bind to the protein encoded by gene X on western blots. You extract DNA and protein from the four strains described above and do southern, northern, and western blot analyses to compare gene X. its mRNA transcript, and its protein in these strains. Please draw the bands you expect to see. Hie positions of the wild type bands are indicated.
Suppose you are studying gene X in yeast using four different strains. Strain 1 is haploid and has the wild type allele of gene X. Strain 2 is haploid and has a missense mutation halfway through the coding sequence of gene X. Strain 3 is haploid and has a nonsense mutation halfway through the coding sequence of gene X. Strain 4 is diploid and is heterozygous for the nonsense mutation in gene X (i.e.. it carries the wild type allele of gene X on one chromosome and the nonsense allele of gene X on the other homologue). You have a DNA probe that will hybridize with gene X and its mRNA transcript on southern and northern blots, respectively. You also have an antibody that will bind to the protein encoded by gene X on western blots. You extract DNA and protein from the four strains described above and do southern, northern, and western blot analyses to compare gene X. its mRNA transcript, and its protein in these strains. Please draw the bands you expect to see. Hie positions of the wild type bands are indicated.
