Question: The constitutive promoter that you placed upstream of your gene
is expressing too much of the gen…
The constitutive promoter that you placed upstream of your gene
is expressing too much of the gene. Which of the following can you
use to have it express less?
☐ Make the promoter weaker by changing
the sequences of the -10 and -35 regions to their reverse
complements
☐ Make the promoter stronger by
changing the sequences of the -10 and -35 regions to more closely
match the consensus sequences
☐ Make the promoter weaker by changing
the sequences of the -10 and -35 regions to more closely match the
consensus sequences
☐ Make the promoter stronger by
changing the sequences of the -10 and -35 regions to less closely
match the consensus sequences
☐ Make the promoter weaker by changing
the sequences of the -10 and -35 regions to less closely match the
consensus sequences