Question: The E. coli Lac operon produces a polycistronic mRNA, an mRNA
with three protein-coding regions (…
The E. coli Lac operon produces a polycistronic mRNA, an mRNA
with three protein-coding regions (see the diagram below). How do
cells handle the problem of the translation termination of the
LacZ-coding region and the translation initiation of the
LacY-coding region? (Note that the LacZ stop codon (UAG) is just
upstream of the LacY start codon (AUG). LacY and LacA do not have
their own S-D sequences) 5’—S-D—AUG-(LacZ)-UAG AUG- (LacY)-UAA
AUG-(LacA)-UAA- 3’UTR
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