Question: The figure below is a called a heatmap, and it is similar to Fig. 18.21 in your book. In this exp…
Show transcribed image text The figure below is a called a heatmap, and it is similar to Fig. 18.21 in your book. In this experiment, amoebae were exposed to four different species of bacteria: Klebsiella pneumoniae ("K.p."), Pseudomonas aeruginosa ("Pa"), Staphylococcus aureus ("S.a."), and Bacillus subtilis ("B.s."). Two of these bacteria are Gram-negative (K.p. and Pa.; in red, on the left) and two are Gram-positive (S.a. and B.s.; in blue, on the right). Immediately upon exposure to the bacteria, the amoebae were harvested and their mRNA was extracted, converted to cDNA, and then sequenced. Each column depicts the transcriptional response of the amoebae to different bacterial species. Each row represents the transcriptional response of a different amoeba gene. Yellow means that transcription of that amoeba gene increased significantly when the amoebae were exposed to that bacterial species. Blue means that transcription that amoeba gene was significantly reduced when exposed to that bacterial species. Numbers indicate how many total genes were upregulated in response to Gram-negative versus Gram-positive bacteria. (Figure from Nasser et al. 2013. Current Biology 23:862-872) Based on these results, choose the statement(s) that accurately describe conclusions from this experiment. Choose all that apply. The experiment shows that a different set of genes is activated when amoebae are exposed to Gram-negative bacteria, compared to when they are exposed to Gram-positive bacteria. The experiment shows that the same genes are always activated in amoebae, regardless of which bacterial species they are exposed to. Fewer genes were upregulated upon exposure to Gram-negative compared to Gram-positive bacteria. More genes were upregulated upon exposure to Gram-negative than Gram-positive bacteria. Amoebae do not change their gene expression depending on the bacterial species they encounter.
The figure below is a called a heatmap, and it is similar to Fig. 18.21 in your book. In this experiment, amoebae were exposed to four different species of bacteria: Klebsiella pneumoniae ("K.p."), Pseudomonas aeruginosa ("Pa"), Staphylococcus aureus ("S.a."), and Bacillus subtilis ("B.s."). Two of these bacteria are Gram-negative (K.p. and Pa.; in red, on the left) and two are Gram-positive (S.a. and B.s.; in blue, on the right). Immediately upon exposure to the bacteria, the amoebae were harvested and their mRNA was extracted, converted to cDNA, and then sequenced. Each column depicts the transcriptional response of the amoebae to different bacterial species. Each row represents the transcriptional response of a different amoeba gene. Yellow means that transcription of that amoeba gene increased significantly when the amoebae were exposed to that bacterial species. Blue means that transcription that amoeba gene was significantly reduced when exposed to that bacterial species. Numbers indicate how many total genes were upregulated in response to Gram-negative versus Gram-positive bacteria. (Figure from Nasser et al. 2013. Current Biology 23:862-872) Based on these results, choose the statement(s) that accurately describe conclusions from this experiment. Choose all that apply. The experiment shows that a different set of genes is activated when amoebae are exposed to Gram-negative bacteria, compared to when they are exposed to Gram-positive bacteria. The experiment shows that the same genes are always activated in amoebae, regardless of which bacterial species they are exposed to. Fewer genes were upregulated upon exposure to Gram-negative compared to Gram-positive bacteria. More genes were upregulated upon exposure to Gram-negative than Gram-positive bacteria. Amoebae do not change their gene expression depending on the bacterial species they encounter.
