Question: The figure of plasmid pBR322 in the Pre-class Tutorial shows that a plasmid vector must have an …
Show transcribed image text The figure of plasmid pBR322 in the Pre-class Tutorial shows that a plasmid vector must have an origin of replication so that it can be copied whenever the cell divides, a useful vector must have several restriction enzyme recognition sites, the restriction recognition sites are in the middle of the reporter genes. a vector must have some reporter genes, so that researchers can determine which cells have picked up the recombinant plasmid. Restriction enzymes create "sticky ends" of DNA, due to overlapping sections that can base-pair. don't harm the bacterial DNA because it doesn't have palindromic sequences, cut at palindromic sequences in DNA. evolved in bacteria to help them defend themselves against invading viral DNA. Once a recombinant DNA molecule has been made, scientists need to get it into a cell. Which of the following statements is/are correct about this topic? A "vector" is a delivery system to get the recombinant DNA into the cell. Plasmids (small, nonchromosomal, circular bacterial DNA molecules) are often engineered to be used as vectors. Viruses can be used as vectors, and can carry larger loads of DNA than plasmids can. A bacterial chromosome is often the vector.
The figure of plasmid pBR322 in the Pre-class Tutorial shows that a plasmid vector must have an origin of replication so that it can be copied whenever the cell divides, a useful vector must have several restriction enzyme recognition sites, the restriction recognition sites are in the middle of the reporter genes. a vector must have some reporter genes, so that researchers can determine which cells have picked up the recombinant plasmid. Restriction enzymes create "sticky ends" of DNA, due to overlapping sections that can base-pair. don't harm the bacterial DNA because it doesn't have palindromic sequences, cut at palindromic sequences in DNA. evolved in bacteria to help them defend themselves against invading viral DNA. Once a recombinant DNA molecule has been made, scientists need to get it into a cell. Which of the following statements is/are correct about this topic? A "vector" is a delivery system to get the recombinant DNA into the cell. Plasmids (small, nonchromosomal, circular bacterial DNA molecules) are often engineered to be used as vectors. Viruses can be used as vectors, and can carry larger loads of DNA than plasmids can. A bacterial chromosome is often the vector.
