Two nucleotide-pair substitutions occur about 10 nucleotides upstream of the +1 site of a bacterial gene during DNA synthesis

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Two nucleotide-pair substitutions occur about 10 nucleotides upstream of the +1 site of a bacterial gene during DNA synthesis and are not repaired. What is the most likely impact of these mutations on transcription? RNA polymerase will bind more strongly to the DNA template, increasing transcription efficiency. The RNA transcript will be unable to form hairpin structures, and thus transcription will not be terminated. The sigma factor and RNA polymerase are less likely to identify the start site of transcription. The mutation will be present in the resulting RNA transcript.
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