Question: Upon light illumination, the photosynthetic electron transport chain of chloroplast is capable of…
Show transcribed image text Upon light illumination, the photosynthetic electron transport chain of chloroplast is capable of transporting [H^+] into the lumen of the chloroplast vesicle to form a proton gradient. The reaction is best observed at pH 6.2 When the light is turned off, the proton gradient will decay and dissipated. Addition of 1 mM pyridine (structure showed in the following) to the reaction mixture leads to a 10-fold increase of the proton gradient and a substantial slow down of the decay of the proton gradient in the dark. Explain the effect of pyridine on the proton translation in chloroplast.
Upon light illumination, the photosynthetic electron transport chain of chloroplast is capable of transporting [H^+] into the lumen of the chloroplast vesicle to form a proton gradient. The reaction is best observed at pH 6.2 When the light is turned off, the proton gradient will decay and dissipated. Addition of 1 mM pyridine (structure showed in the following) to the reaction mixture leads to a 10-fold increase of the proton gradient and a substantial slow down of the decay of the proton gradient in the dark. Explain the effect of pyridine on the proton translation in chloroplast.
