Question: When the gene encoding certain cytoskeleton protein is
deleted, the resulting mutant cells round …
When the gene encoding certain cytoskeleton protein is
deleted, the resulting mutant cells round up and do not form their
normal appendages. These mutants can be rescued when a gene
encoding an N-terminal green fluorescent protein (GFP) fusion of
the protein is expressed, but not when a gene encoding a C-terminal
GFP fusion is expressed. Which fusion protein (N or C) is
appropriate to use in studying cellular localization and activity?
Write down N or C as your answer and explain.
deleted, the resulting mutant cells round up and do not form their
normal appendages. These mutants can be rescued when a gene
encoding an N-terminal green fluorescent protein (GFP) fusion of
the protein is expressed, but not when a gene encoding a C-terminal
GFP fusion is expressed. Which fusion protein (N or C) is
appropriate to use in studying cellular localization and activity?
Write down N or C as your answer and explain.
(Visited 2 times, 1 visits today)
