Question: Wild-type strains of E, coil have a tRNA with a 5′-GCA-3′ anticodon that recognizes the UGC codon…
Show transcribed image text Wild-type strains of E, coil have a tRNA with a 5'-GCA-3' anticodon that recognizes the UGC codon for cysteine. We can represent this tRNA as GCA-tRNA^Cys. Suppose you inclined a strain of E, coil in which a mutation changed the anticodon in this tRNA to 5^'-GCA-3^' (creating UCA-tRNA^Cys). Answer the following questions about translation alongation in each strain. For each active type in "yes" or "no". Will the wildtypetRNA recognize a normal UGC codon in the A site? Will the mutanttRNA recognize a normal UGC codon in the A site?Will the wildtypetRNA recognize a stop codon in the A site?Will the mutant tRNA recognize stop codon in the A site? Will peptide bond formation occur with the wildtypetRNA bound in the A site? Will peptide bond formation occur with the mutanttRNA bound in the A site? Will the normal, wildtype protein be produced in the wildtype strain? Will the normal, wildtype protein be produced in the mutant strain?
Wild-type strains of E, coil have a tRNA with a 5'-GCA-3' anticodon that recognizes the UGC codon for cysteine. We can represent this tRNA as GCA-tRNA^Cys. Suppose you inclined a strain of E, coil in which a mutation changed the anticodon in this tRNA to 5^'-GCA-3^' (creating UCA-tRNA^Cys). Answer the following questions about translation alongation in each strain. For each active type in "yes" or "no". Will the wildtypetRNA recognize a normal UGC codon in the A site? Will the mutanttRNA recognize a normal UGC codon in the A site?Will the wildtypetRNA recognize a stop codon in the A site?Will the mutant tRNA recognize stop codon in the A site? Will peptide bond formation occur with the wildtypetRNA bound in the A site? Will peptide bond formation occur with the mutanttRNA bound in the A site? Will the normal, wildtype protein be produced in the wildtype strain? Will the normal, wildtype protein be produced in the mutant strain?
