Question: You are sequencing a PCR product from the Bluease gene. The
sequence of the region and the sequen…
You are sequencing a PCR product from the Bluease gene. The
sequence of the region and the sequence of the oligonucleotide you
are using are shown here. DNA:
5′-ATACGCAGACTTCGAGCGCCCACAC-3′
3′-TATGCGTCTGAAGCTCGCGGGTGTG-5′
Primer: 5′-TTCGA-3′
Draw what the chromatograph will look like after you have used
this oligo to sequence this piece of DNA.
A T C G
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