Question: You generate a set of Glutamine auxotrophs. Using complementation analysis, you identify 3 critic…

You generate a set of Glutamine auxotrophs. Using
complementation analysis, you identify 3 critical genes in the
biochemical pathway for Glutamine, and isolate clones of single and
double mutants for these genes.

When feeding the clones with minimal medium, various biochemical
precursors (), or Glutamine, you observe the
survival behavior presented in the table below (+ means survival).
Use an analysis of epistatis to
determine the order of the biochemical pathway (showing the order
of the precursors as well as the enzymes encoded by the genes A, B,
and C, similar to what we saw in class).