Question: You have made a specific DNA probe that will bind to a key sequence on the DNA of Nesseria gonorr…

You have made a specific DNA probe that will bind to a key
sequence on the DNA of Nesseria gonorrhoeae, allowing your company
to market a gonorrhea test kit that can be used to identify the
bacterium in genital tract specimens. However, upon testing it out
against a known Neisseria gonorrhoeae culture, you find that it
does not work. Which of the following is a possible explanation for
this negative result?

A.You forgot to label the probe sequence with a reporter
molecule.

B. You forget to digest the probe with restriction
endonucleases

C. You forget to add the mRNA to the DNA probe sample.

D. You forgot to incubate the test at the 75C temperature
required for hybridization.