Question: You transfer 1 ml. from a bacterial suspension with 50%
viability into 99 ml. diluent, then sprea…
You transfer 1 ml. from a bacterial suspension with 50%
viability into 99 ml. diluent, then spread 0.1 ml. from the
resultant suspension onto a petri dish, to yield 50 colonies. The
original suspension contained ____ total bacteria per ml.
You transfer 10 microliters from a bacterial suspension into
99.99 ml. sterile diluent and plate 0.1 ml. to get 100 colonies on
your spread plate. How many viable bacteria per ml. were there in
the original suspension?
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