Question: You wish to make a recombinant DNA molecule that will contain one piece of pKABOO vector DNA and …
You wish to make a recombinant DNA molecule
Show transcribed image text You wish to make a recombinant DNA molecule that will contain one piece of pKABOO vector DNA and one piece of frog DNA so that you can clone a segment of frog DNA. You want cells containing your recombinant plasmid to be amp and let and you want to use enzymes that cut within the insertion marker gene. (Note that let means that there is no functional let gene in the plasmid) Be sure that your plasmid has the ability to replicate autonomously in a bacterial cell. You do not have to include the entire frog DNA given below in your recombinant plasmid. Restriction enzymes would be used to clone a segment of frog DNA. The size of the recombinant plasmid is bp. The recombinant plasmid when transformed into E. coli confers resistance to which of the following antibiotics: beta-galactocillinonly ampicillin and tetracycline ampicillin only tetracycline only ampicillin and beta-galactocillin tetracycline and beta-galacatocillin The recombinant plasmid map has many restrictio
You wish to make a recombinant DNA molecule that will contain one piece of pKABOO vector DNA and one piece of frog DNA so that you can clone a segment of frog DNA. You want cells containing your recombinant plasmid to be amp and let and you want to use enzymes that cut within the insertion marker gene. (Note that let means that there is no functional let gene in the plasmid) Be sure that your plasmid has the ability to replicate autonomously in a bacterial cell. You do not have to include the entire frog DNA given below in your recombinant plasmid. Restriction enzymes would be used to clone a segment of frog DNA. The size of the recombinant plasmid is bp. The recombinant plasmid when transformed into E. coli confers resistance to which of the following antibiotics: beta-galactocillinonly ampicillin and tetracycline ampicillin only tetracycline only ampicillin and beta-galactocillin tetracycline and beta-galacatocillin The recombinant plasmid map has many restrictio
