Question: You wish to make a recombinant DNA molecule that will contain one piece of pKABOO vector DNA and …

You wish to make a recombinant DNA molecule that will contain
one piece of pKABOO vector DNA and one piece of frog DNA so that
you can clone a segment of frog DNA. You want cells containing your
recombinant plasmid to be amp^r and tet^s and
you want to use enzymes that cut within the insertional marker
gene. (Note that tet^s means that there is no functional
tet^r gene in the plasmid.) Be sure that your plasmid has
the ability to replicate autonomously in a bacterial cell. You do
not have to include the entire frog DNA given below in your
recombinant plasmid.

Restriction enzymes AnswerSpeI and NotIEcoRI and NotIBamHI and
NotIEcoRI and SpeIEcoRI and XmaIIIBamHI and XmaIIINotI and
XmaIIISpeI and XmaIIIEcoRI and BamHIBamHI and SpeIEcoRI only would
be used to clone a segment of frog DNA.

The size of the recombinant plasmid is

The recombinant plasmid when transformed into E. coli
confers resistance to which of the following antibiotics:

tetracycline and beta-galactocillin

tetracycline only

beta-galactocillin only

ampicillin and beta-galactocillin

ampicillin only

ampicillin and tetracycline

The recombinant plasmid map has many restriction sites.
Determine the base pair position of each of the following sites in
the recombinant plasmid map: