Question: Your graduate advisor asks you to amplify the following sequence
of DNA by PCR:
a) To ensure tha…
Your graduate advisor asks you to amplify the following sequence
of DNA by PCR:
a) To ensure that the entire sequence above is amplified, what
6-nucleotide DNA primers should you add to your PCR mix?
You order the primers listed above, but instead receive the
following set of primers:
b) What portion of the double
stranded DNA molecule will be amplified after 10 rounds of
PCR?
Your labmate attempts to rescue your PCR reaction by providing
you with the following set of primers:
c. What is the result of running the PCR reaction with your
labmate’s primers? How many double stranded molecules of DNA will
result from 10 rounds of amplification?
